HISTOLOGICAL AND HISTOCHEMICAL STUDIES ON THE FAT BODY IN CULEX AFTER TREATMENT WITH A NEWLY SYNTHESIZED JHA

The fourth instar larvae of Culex pippins quinquefasciatus (24 h old) were reared in sublethal doses (1, 2 and 3 ppm) of a newly synthesized JHA : 1-(3'-methyl-6-isopropylcyclohexyloxy-3, 7-dimethyl-2(E), 6octadiene for 24 h. Their histological and histochemical studies revealed that JHA treatment produced decrease in number of fat body cells, vacuoles in them and change to their cellular membranes. It was dose dependent. DNA, RNA, proteins, 1:2 glycol groups, lipids (acidic and neutral) decreased qualitatively whereas glycogen showed an increase. These changes may be responsible for larval mortality and incomplete moulting after JHAtreatment.