ANTI BACTERIAL ACTIVITY OF Cordia dichotoma G. FORST. BARK HEXANE EXTRACT AND IDENTIFICATION OF ITS CONSTITUENTS BY GC-MS ANALYSIS

SWAPNA, GONE and SHANKER, KAKI SHIVA and AZMEERA, THIRUPATHI and RANI, G. USHA and RAO, BHATTIPROLU KESAVA (2020) ANTI BACTERIAL ACTIVITY OF Cordia dichotoma G. FORST. BARK HEXANE EXTRACT AND IDENTIFICATION OF ITS CONSTITUENTS BY GC-MS ANALYSIS. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21 (63). pp. 55-62.

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Abstract

Aim: Cordia dichotoma G. Forst belongs to the family Boraginaceae is known for its biological activities. The plant stem bark powder has been used to treat ulcers. The stem bark has anti-microbial and anti-implantation activities. The present study aims to analyse the phytoconstituents of stem bark hexane extract by GC-MS analysis, and to investigate its antibacterial activity.

Methods: The powdered stem bark was extracted with cold and hot hexane solvent and then the solvent was distilled to check its constituents by GCMS. The crude hexane was further purified by saponification, Column chromatography, and preparative TLC. The Fractions obtained from the above-mentioned methods were analyzed by GCMS. The hexane extract was further evaluated for antibacterial activity using two bacterial pathogens by the disc diffusion method.

Results: From GCMS analysis, eleven components were identified from crude hexane extract, eight components from Fraction 16c, and three components from Fraction 16d based on peak area percentage, retention time, and structure.

Item Type: Article
Subjects: Asian STM > Biological Science
Depositing User: Managing Editor
Date Deposited: 04 Dec 2023 03:55
Last Modified: 04 Dec 2023 03:55
URI: http://journal.send2sub.com/id/eprint/2871

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