Chemical Characterization of Cinnamomum tamala Leaves by HPLC Analysis

Cinnamomum tamala (CT) is the member of Lauraceae family and its leaves are most popularly used as spice in Indian food. Along with spice, it is associated with many medicinal values such as antioxidant, antidiabetic, antifungal, immunomodulation etc. On the basis of GC-MS analysis, Eugenol was reported as active constituent who is present in excess in non polar fraction. Here, the study about the qualitative analysis of different phytochemicals in defferent fractions of CT leaves along with their TLC fingerprinting. Phytochemical analysis was carried out by chemical reaction based and quantitative estimation of eugenol was done by High Performance Liquid Chromatography (HPLC) using C-18 column. Result shown that the distribution of different phytochemicals were varies in different fraction and maximum amount of eugenol was found in non polar fraction (Hexane fraction) and then in total methanol fraction. It was found in traces in successive ethyl acetate and methanol fraction.