Study on the Evaluation of Hepatoprotective Activity with Leaf Extract of Alangium salvifolium Wang on CCl4-Induced Wistar Albino Rats

Liver injury is mainly caused by toxic chemicals, excess consumption of alcohol, infections and autoimmune disorders. Most hepatotoxic chemicals damage liver cells mainly by inducing lipid peroxidation and other oxidative damage. The present study aimed at Evaluating Hepatoprotective Activity with Leaf Extract of Alangium salvifolium Wang (Alangiaceae) on CCl4 induced Rats. Traditionally this plant is used in various types of liver disorders and is also used as an antidiabetic, anticancer, diuretic, cardiotonic, anti-inflammatory, antimicrobial, Laxative, epilepsy, antidote for poisonings, burning sensation, constipation and haemorrhages.

The leaves of Alangium salvifolium were collected and prepared as ethanolic extracts of Alangium salvifolium (ASEE), Silymarin, Ethanol, and CCl4, CMC, Olive oil, analytical kits from standard companies. Albino Wistarrats(150-180 g) from TEENA Labs.

An acute toxicity study was conducted in animals to identify of LD50 value. Two test doses were selected on the basis of LD50 and conducted Hepatoprotective activity by using the CCl4-induced method. The collected blood samples were subjected to various biochemical parameters (SGPT, SGOT, ALP, TB, ALB, TP and CHOL). Dissected the liver for histopathological studies. The data were statistically assessed by one-way analysis of variance (ANOVA) and subjected to Dunnett’s test.

In hepatoprotective activity studies, the toxic group (CCl4) shows elevated levels of SGPT, SGOT, ALP, CHOL and TBL whereas decreased TP and ALB levels when compared with the control group. The pretreatment of ASEE at a dose of 150 and 300 mg/kg exhibited a reduction in the serum levels of SGPT(P<0.001), SGOT(P<0.001), ALP(P<0.01), CHOL(P<0.01), TB(P<0.001), and elevated the TP(P<0.01), ALB(P<0.001) levels when compared to toxic. ASEE of 300 mg/kg showed results similar to standard results. Histopathological studies revealed that ASEE exhibited a remarkable recovery as Silymarin does. The results of this study strongly indicate that leaves of Alangium Salvifolium have potent hepatoprotective against CCl4. The hepatoprotective activity of ASEE may be due to its free radical-scavenging and antioxidant activity, resulting from the presence of flavonoids and triterpenoid compounds in the extracts.