Evaluation of Acute Oral Toxicity of Ayucee Premix Polyherbal Poultry Formulation Containing Natural Vitamin C and Bioflavonoids

A study was conducted for the assessment of acute oral toxicity and safety analysis of Ayucee Premix (M/s Ayurvet Limited Baddi, India) according to OECD-423 guidelines. Six young adult Wistar rats, weighing 126-136 gm, were used for the study. After Oral administration of test compound the animals were observed for manifestation of toxic effect and death. Each animal served as its own control. Evaluation of safety analysis and toxicity was performed on the basis of presence of mortality or any clinical signs like lethargy, tremor, abdominal breathing and piloerection. Results showed no toxic effects or mortality during the experimental period. In blood biochemical parameters (AST, ALT, ALP) and histopathological examination no significant pathological alterations were observed. Thus, Ayucee Premix was found to be safe for oral consumption.